First, he had taken leaves of individuals of 3 species (Virola Mickaelii, Qualea Rosea and Eperua grandifolia) trees to measure their "deltaC13.
Being a bit lazy, I'll copy part of a small summary, by transferring the forms and everything that's going a bit too much detail:
Delta C13 results from a balance between the action of rubisco that discriminates and that if she was alone in the game would fix around 29 ‰, and the diffusion of carbon 13 that if it was only fixes the rate-limiting delta C13 to 4.4 ‰.
Its value tells us about the character thus limiting the diffusion of carbon 13, and is well correlated with WUE is defined as the ratio of the rate of CO2 uptake by leaf area (A) conductance stomach for the vapor water (gs). The WUE is used to account for the efficiency of water use of a plant.
This is achieved by mass spectrometry in the gas phase of 1 mg of dried leaves of light at 70 ° C for 48 h and crushed at least 50 microns.
There is a local gradient of carbon 13, and isotopic composition of air varies over time. Also assumptions may be questionable, the isotopic signature sheets could depend on controllable environmental variables, including the exposure of leaves taken, and their date of establishment. It
n'échantillonne only individuals in the shade, CP = 1 (index Dawkins, 1 is for trees completely covered under, who do not receive direct light) to limit the effect of illumination; should be checked a posteriori the equivalence of exposure between branches taken in ensuring that the variability within the LMA series is over the variability between series, the branches are removed at a constant height on individuals of the same size growing out shoal.
was sampled along the leaf series (on an axis relative age known)
For each series we estimate an intra-and inter UC UC UC to test the effect
There is always the largest insertion of leaves collected (numbering basipetal ). It describes each branch (in length, limits likely ua if any) and basic information of the plant on which the branch is taken will be recorded (position, height, number of branches / points growing number floors where applicable).
So here I am in mop to find branches of individuals of these three species, only in the shade, not in a shallow, if not for the seasonal variability is damn (he's standing in water throughout the year) so rather plateau, on sandy soil ...
He must have a minimum number of CPUs.
A growth unit is a set length of stem, leaves, etc. ... implemented at once. Between two CPUs, there is a stop of growth more or less materialized by a scar, a shorter inter-nodes, a small pair of leaves etc ... etc ...
is important because interest take several sheets in a single CPU is low if it is implemented in a single instance.
And above all: off-plot, because there is no question of going massacrerdes young trees on the plots studied ... It, of course, that means going outside the transects wandering ... So
«remake, I'm out and about in search of stuff still rather quiet, I need to measure before they take a branch that I was back home for analysis. Accompanied by little of course: it's the holidays, apart from students, there are not many people in the lab.
is an opportunity to meet people.
agoutis, some sparrows, a coral snake on which I failed to walk a huge Grage (a snake too) ... many animals you can not see, or just as it is too many.
So I brought my branches, it remains to carefully measure all lengths between nodes, the length, width, leaf area ...
The beard ... the repetitive possible, using ... boring. A real work of an intern.
But I have a camera, tripod, a large cardboard box ... I will buy colored pins, graph paper ... and I'll do the photometry!
is true after all, so much to measure more ... while on a photo well done, you can do everything at once!
So the device:
The pins hold the green stem and leaves stretched, red pins mark a leaf scar, and yellow pins are spaced 5cm and can deliver all scale taking into account the deformation related to the photo.
Then he had to write a small program to process these images, a program that isolates the leaves, class and measure their area, find the nodes, and measuring the areas between the nodes, and gathers all ... A
After all this effort is, my supervisor decided to take the opportunity to see if there is a correlation between different markers in UA Qualea: the length between nodes, and using the leaves. In addition to allowing us to pinpoint the CPU, this could be the basis for a model calculating the leaf area from the lengths of internodes ... more need to measure the leaves on the ground (which is very tedious), a simple measure of length would have been enough! So
graphics outputs: Virola on first;
must initially thresholded image. Not very complicated ... The program recorks holes sheets etc. ...
And now, the program has now been extracted from the bottom branch, and has redéformé taking account of the yellow markers.
Hop, he found the petioles ... and some that will remove parasites.
That leaves, and their range.
The branch ...
The branch and its nodes ...
The branch, its nodes, and lengths of internodes!
Soon, the same treatment on Qualea Rosea, with a small additional subtlety; Qualea has opposite leaves alternate and not as Virola ...
And then, the results of the analysis ...
Suspense; markers proposed by UC Sanoja Are these neat young twigs Qualea? Are visible and coherent? Can we deduct the leaf surfaces of the lengths of-noueds??
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